sc 136480  (Santa Cruz Biotechnology)

Journal: International journal of molecular sciences

Article Title: Non-Invasive Assessment of Neurogenesis Dysfunction in Fetuses with Early-Onset Growth Restriction Using Fetal Neuronal Exosomes Isolating from Maternal Blood: A Pilot Study.

doi: 10.3390/ijms26041497

Figure Lengend Snippet: Figure 1. (A) Western blot of the membrane. The membranes represent a set of three samples from each group. (B) Comparative analysis of proteins in FNE from pregnant women with early-onset FGR (n = 8) and comparison group (CG) (n = 8). Data are presented in the format Me (Q1, Q3); *: significance level p ≤0.05, **: significance level p ≤0.01, ***: significance level p ≤0.001, when compared with CG. SYT1—Synaptotagmin 1, SYN1—Synapsin 1, SYP—Synaptophysin, STX1— Syntaxin 1, and SYNPO—Synaptopodin.

Article Snippet: The membranes were blocked with 5% NFDM/TBST for 2 h for subsequent incubation during an hour at the room temperature with primary antibodies, including BDNF (UniProt: P23560) (1:1000, ab205067, Abcam, Boston, MA, USA), NGF (UniProt: P01138) (1:1000, SI79-01 (rabbit), Thermo Fisher Scientific, USA), Synaptotagmin 1 (SYT1) (UniProt: P21579) (1:200, sc136480, Santa Cruz Biotechnology, Dallas, TX, USA), Synapsin 1 (SYN1) (UniProt: P17600) (1:1000, ab254349 (rabbit), Abcam, USA), Synaptophysin (SYP) (UniProt: P08247) (1:1000, sc-17750, Santa Cruz Biotechnology, USA), Syntaxin 1 (STX1) (UniProt: Q16623) (1:200, sc-12736, Santa Cruz Biotechnology, USA), Synaptopodin (SYNPO) (UniProt: Q8N3V7) (1:100, sc-515842, Santa Cruz Biotechnology, USA), PSD95 (UniProt: P78352) (1:200, sc32291, Santa Cruz Biotechnology, USA), Neuronal Pentraxin 2 (NPTX2) (UniProt: P47972) (1:1000, ab277523 (rabbit), Abcam, USA), NEDD8 (UniProt: Q15843) (1:1000, ab81264, (rabbit), Abcam, USA), APPBP1 (NAE1) (UniProt: Q13564) (1:100, sc-390002, Santa Cruz Biotechnology, USA), UBC12 (UniProt: P61081) (1:100, sc-390064, Santa Cruz Biotechnology, Int.

Techniques: Western Blot, Membrane, Comparison

Journal: International journal of molecular sciences

Article Title: Non-Invasive Assessment of Neurogenesis Dysfunction in Fetuses with Early-Onset Growth Restriction Using Fetal Neuronal Exosomes Isolating from Maternal Blood: A Pilot Study.

doi: 10.3390/ijms26041497

Figure Lengend Snippet: Figure 2. Spearman’s correlation matrix obtained from the analysis of correlations between pro- tein expression in FNE and clinical parameters in early-onset FGR. The diameter of the circles and the color indication (according to the scale on the right) are proportional to the correla- tion coefficient; the fields where statistical significance of the correlation coefficients is greater than 0.05. SYT1—Synaptotagmin 1, SYN1—Synapsin 1, SYP—Synaptophysin, STX1—Syntaxin 1, SYNPO—Synaptopodin, FW—newborn weight, H-C—heel-crown, WP—weight percentile, WP (US)—weight percentile (ultrasound), BD—biparietal diameter of the fetus, HC—head circumfer- ence, AC—abdominal circumference, EFW—estimated fetal weight, PI UtA.s.—Pulsatility index of Uterine Artery (left), PI UtA.d.—Pulsatility index of uterine artery (right), PI UA—Pulsatility index of umbilical artery, PI MCA—Pulsatility index of middle cerebral artery, and CPR—cerebral placental ratio.

Article Snippet: The membranes were blocked with 5% NFDM/TBST for 2 h for subsequent incubation during an hour at the room temperature with primary antibodies, including BDNF (UniProt: P23560) (1:1000, ab205067, Abcam, Boston, MA, USA), NGF (UniProt: P01138) (1:1000, SI79-01 (rabbit), Thermo Fisher Scientific, USA), Synaptotagmin 1 (SYT1) (UniProt: P21579) (1:200, sc136480, Santa Cruz Biotechnology, Dallas, TX, USA), Synapsin 1 (SYN1) (UniProt: P17600) (1:1000, ab254349 (rabbit), Abcam, USA), Synaptophysin (SYP) (UniProt: P08247) (1:1000, sc-17750, Santa Cruz Biotechnology, USA), Syntaxin 1 (STX1) (UniProt: Q16623) (1:200, sc-12736, Santa Cruz Biotechnology, USA), Synaptopodin (SYNPO) (UniProt: Q8N3V7) (1:100, sc-515842, Santa Cruz Biotechnology, USA), PSD95 (UniProt: P78352) (1:200, sc32291, Santa Cruz Biotechnology, USA), Neuronal Pentraxin 2 (NPTX2) (UniProt: P47972) (1:1000, ab277523 (rabbit), Abcam, USA), NEDD8 (UniProt: Q15843) (1:1000, ab81264, (rabbit), Abcam, USA), APPBP1 (NAE1) (UniProt: Q13564) (1:100, sc-390002, Santa Cruz Biotechnology, USA), UBC12 (UniProt: P61081) (1:100, sc-390064, Santa Cruz Biotechnology, Int.

Techniques: Expressing

Journal: International journal of molecular sciences

Article Title: Non-Invasive Assessment of Neurogenesis Dysfunction in Fetuses with Early-Onset Growth Restriction Using Fetal Neuronal Exosomes Isolating from Maternal Blood: A Pilot Study.

doi: 10.3390/ijms26041497

Figure Lengend Snippet: Figure 3. Changes in FNE protein expression in newborns with intrauterine pneumonia (IP), respi- ratory distress syndrome (RDS) and pulmonary hypertension (PH), depending on the presence of the disorder (1) or its absence (0). Data are presented in the format Me (Q1, Q3); *: significance level p ≤0.05. **: significance level p ≤0.01. ***: significance level p ≤0.001. SYT1—Synaptotagmin 1, SYN1—Synapsin 1, SYP—Synaptophysin, STX1—Syntaxin 1, and SYNPO—Synaptopodin.

Article Snippet: The membranes were blocked with 5% NFDM/TBST for 2 h for subsequent incubation during an hour at the room temperature with primary antibodies, including BDNF (UniProt: P23560) (1:1000, ab205067, Abcam, Boston, MA, USA), NGF (UniProt: P01138) (1:1000, SI79-01 (rabbit), Thermo Fisher Scientific, USA), Synaptotagmin 1 (SYT1) (UniProt: P21579) (1:200, sc136480, Santa Cruz Biotechnology, Dallas, TX, USA), Synapsin 1 (SYN1) (UniProt: P17600) (1:1000, ab254349 (rabbit), Abcam, USA), Synaptophysin (SYP) (UniProt: P08247) (1:1000, sc-17750, Santa Cruz Biotechnology, USA), Syntaxin 1 (STX1) (UniProt: Q16623) (1:200, sc-12736, Santa Cruz Biotechnology, USA), Synaptopodin (SYNPO) (UniProt: Q8N3V7) (1:100, sc-515842, Santa Cruz Biotechnology, USA), PSD95 (UniProt: P78352) (1:200, sc32291, Santa Cruz Biotechnology, USA), Neuronal Pentraxin 2 (NPTX2) (UniProt: P47972) (1:1000, ab277523 (rabbit), Abcam, USA), NEDD8 (UniProt: Q15843) (1:1000, ab81264, (rabbit), Abcam, USA), APPBP1 (NAE1) (UniProt: Q13564) (1:100, sc-390002, Santa Cruz Biotechnology, USA), UBC12 (UniProt: P61081) (1:100, sc-390064, Santa Cruz Biotechnology, Int.

Techniques: Expressing

Journal: International journal of molecular sciences

Article Title: Non-Invasive Assessment of Neurogenesis Dysfunction in Fetuses with Early-Onset Growth Restriction Using Fetal Neuronal Exosomes Isolating from Maternal Blood: A Pilot Study.

doi: 10.3390/ijms26041497

Figure Lengend Snippet: Figure 4. Changes in FNE protein expression in newborns with cerebral ischemia, IVH, asphyxia and CNS depression syndrome, depending on the presence of the disorder (1) or its absence (0). Data are presented in the format Me (Q1, Q3); *: significance level p ≤0.05. **: significance level p ≤0.01. ***: significance level p ≤0.001. SYT1—Synaptotagmin 1, SYN1—Synapsin 1, SYP—Synaptophysin, STX1—Syntaxin 1, and SYNPO—Synaptopodin.

Article Snippet: The membranes were blocked with 5% NFDM/TBST for 2 h for subsequent incubation during an hour at the room temperature with primary antibodies, including BDNF (UniProt: P23560) (1:1000, ab205067, Abcam, Boston, MA, USA), NGF (UniProt: P01138) (1:1000, SI79-01 (rabbit), Thermo Fisher Scientific, USA), Synaptotagmin 1 (SYT1) (UniProt: P21579) (1:200, sc136480, Santa Cruz Biotechnology, Dallas, TX, USA), Synapsin 1 (SYN1) (UniProt: P17600) (1:1000, ab254349 (rabbit), Abcam, USA), Synaptophysin (SYP) (UniProt: P08247) (1:1000, sc-17750, Santa Cruz Biotechnology, USA), Syntaxin 1 (STX1) (UniProt: Q16623) (1:200, sc-12736, Santa Cruz Biotechnology, USA), Synaptopodin (SYNPO) (UniProt: Q8N3V7) (1:100, sc-515842, Santa Cruz Biotechnology, USA), PSD95 (UniProt: P78352) (1:200, sc32291, Santa Cruz Biotechnology, USA), Neuronal Pentraxin 2 (NPTX2) (UniProt: P47972) (1:1000, ab277523 (rabbit), Abcam, USA), NEDD8 (UniProt: Q15843) (1:1000, ab81264, (rabbit), Abcam, USA), APPBP1 (NAE1) (UniProt: Q13564) (1:100, sc-390002, Santa Cruz Biotechnology, USA), UBC12 (UniProt: P61081) (1:100, sc-390064, Santa Cruz Biotechnology, Int.

Techniques: Expressing

Journal: International journal of molecular sciences

Article Title: Non-Invasive Assessment of Neurogenesis Dysfunction in Fetuses with Early-Onset Growth Restriction Using Fetal Neuronal Exosomes Isolating from Maternal Blood: A Pilot Study.

doi: 10.3390/ijms26041497

Figure Lengend Snippet: Figure 5. Changes in FNE protein expression in newborns with thrombocytopenia, DIC syndrome, cutaneous hemorrhage and gastrointestinal bleeding, depending on the presence of the disease (1) or its absence (0). Data are presented in the format Me (Q1, Q3); *: significance level p ≤0.05. **: significance level p ≤0.01. ***: significance level p ≤0.001. DIC syndrome is a disseminated intravascular coagulation. SYT1—Synaptotagmin 1, SYN1—Synapsin 1, SYP—Synaptophysin, STX1— Syntaxin 1, and SYNPO—Synaptopodin.

Article Snippet: The membranes were blocked with 5% NFDM/TBST for 2 h for subsequent incubation during an hour at the room temperature with primary antibodies, including BDNF (UniProt: P23560) (1:1000, ab205067, Abcam, Boston, MA, USA), NGF (UniProt: P01138) (1:1000, SI79-01 (rabbit), Thermo Fisher Scientific, USA), Synaptotagmin 1 (SYT1) (UniProt: P21579) (1:200, sc136480, Santa Cruz Biotechnology, Dallas, TX, USA), Synapsin 1 (SYN1) (UniProt: P17600) (1:1000, ab254349 (rabbit), Abcam, USA), Synaptophysin (SYP) (UniProt: P08247) (1:1000, sc-17750, Santa Cruz Biotechnology, USA), Syntaxin 1 (STX1) (UniProt: Q16623) (1:200, sc-12736, Santa Cruz Biotechnology, USA), Synaptopodin (SYNPO) (UniProt: Q8N3V7) (1:100, sc-515842, Santa Cruz Biotechnology, USA), PSD95 (UniProt: P78352) (1:200, sc32291, Santa Cruz Biotechnology, USA), Neuronal Pentraxin 2 (NPTX2) (UniProt: P47972) (1:1000, ab277523 (rabbit), Abcam, USA), NEDD8 (UniProt: Q15843) (1:1000, ab81264, (rabbit), Abcam, USA), APPBP1 (NAE1) (UniProt: Q13564) (1:100, sc-390002, Santa Cruz Biotechnology, USA), UBC12 (UniProt: P61081) (1:100, sc-390064, Santa Cruz Biotechnology, Int.

Techniques: Expressing, Coagulation